NettetHoechst 33342-induced apoptosis is associated with disruption of TATA box binding protein/TATA box complexes, replication protein A/single-stranded DNA complexes, topoisomerase I/DNA cleavable complexes and with an increased intracellular concentration of E2F-1 transcription factor and nitric oxide concentration. NettetSimilarly, among the three cell lines, MTDH/AEG-1 siRNA had the most significant influence in the T24 cells. The results of Hoechst 33342 and PI double fluorescent staining indicated a clear increase in apoptosis after transfection with MTDH/AEG-1 siRNA, especially at 96 hours (Figures 10D–10F and 11).
2,5-Hexanedione induces human ovarian granulosa cell apoptosis …
NettetHoechst 33342 treatment increased the concentration of E2F-1 protein after a 3-hour incubation in both cell lines. Conclusion: Hoechst 33342-induced apoptosis is … NettetHoechst 33342 and 33258 are quenched by bromodeoxyuridine (BrdU), which is commonly used to detect dividing cells. Hoechst 33342 exhibits a 10 fold greater cell … horseradish fermented
Hoechst 33342 trihydrochloride - MedchemExpress.com
NettetHoechst 33342, a vital DNA-specific fluo-rochrome, was added for determinations of DNA content and subsequent cell cycle analysis (Fig. 1). There is a decrease in the … NettetApproximate fl uorescence excitation/emission maxima, when bound to DNA: ~350/461 nm Hoechst dye; ~535/617 nm propidium iodide Chromatin Condensation/Dead Cell … Nettet18. jun. 2016 · Furthermore, compound 1 dose-dependently induced A2780 cells apoptosis as evidenced by Hoechst 33342 staining, Annexin V positive cells, the up-regulated cleaved-PARP and the enhanced Bax/Bcl-2 ratio. What’s more, compound 1 also promoted the expression of the tumor suppressor p53 protein. horseradish farms